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KMID : 0364820140500010033
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Korean Journal of Microbiology
2014 Volume.50 No. 1 p.33 ~ p.39
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Characteristics of Biosurfactant Producing Pseudomonas sp. HN37
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Jung Da-Hee
Chang Dong-Ho
Kim Yeong-Eun
Jeong Mi-Rang
Han Kyu-Woong
Kim Hyong-Bai
Park Kyeong-Ryang
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Abstract
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One hundred forty four bacterial colonies which were able to degrade crude oil were isolated from soil samples that were contaminated with oil in Daejeon area. Among them, one bacterial strain was selected for this study based on its emulsification activity, growth rate and surface tension activity, and this selected bacterial strain was identified as Pseudomonas sp. HN37 through physiological- biochemical tests and analysis of its 16S rRNA sequence. Pseudomonas sp. HN37 utilize the several aliphatic hydrocarbons, 3,5-dinitrosalicylic acid and 2,4-dichlorophooxyacetic acid as a sole carbon source. And this bacterial strain showed a high resistance to antibiotics such as ampicillin and chloramphenicol, as well as heavy metals such as Ba, Cr, Li and Mn. Also, it was found that the optimal pH and temperature for the cell growth, surface tension, and emulsification activity of Pseudomonas sp. HN37 were pH 6.0?9.0 and 30℃, respectively. The emulsification and surface tension activity was reached the maximum to 1% (V/V) crude oil and 1% (W/V) NaCl concentration. The surface tension of the culture broth was decreased from 62 to 27 dyne/cm after fifteen hours of inoculation in LB media.
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KEYWORD
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Pseudomonas sp. HN37, biosurfactant, colony count, emulsification, surface tension
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